In Vivo Stability of Polyurethane-Based Electrospun Vascular Grafts in Terms of Chemistry and Mechanics

In Vivo Stability of Polyurethane-Based Electrospun Vascular Grafts in Terms of Chemistry and Mechanics Full article

Journal

Polymers
ISSN: 2073-4360

Output data

Year: 2020, Volume: 12, Number: 4, Article number : 845, Pages count : 11 DOI: 10.3390/polym12040845

Tags

tecoflex; pellethane; gelatin; electrospinning; vascular grafts; polyurethane stability in vivo

Authors

Alexander A Gostev ¹ , Shundrina Inna K ^2,3 , Pastukhov Vitaliy I ^2,3 , Shutov Alexey V ^3,5 , Vera S Chernonosova ⁴ , Andrey A Karpenko ¹ , Laktionov Pavel P ^1,4

Affiliations

1	Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation, 630055 Novosibirsk, Russia
2	Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, Russia
3	Novosibirsk State University, ul. Pirogova, 2, 630090 Novosibirsk, Russia
4	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, Russia
5	Lavrentiev Institute of Hydrodynamics, Siberian Branch, Russian Academy of Sciences, 630090 Novosibirsk, Russia

The biostability of the polyurethanes Tecoflex EG-80A and Pellethane 2363-80A, used as basic polymers of the vascular grafts (VGs) produced by electrospinning, as well as the tensile strength of Tecoflex VGs, are studied. Solutions of Tecoflex or Pellethane with gelatin and bivalirudin in 1,1,1,3,3,3-hexafluoroisopropanol are used for VG production. After 1, 12, and 24 weeks of VG implantation in the infrarenal position of the abdominal aorta of Wistar rats, VGs are explanted, fixed in formalin, freed from outer tissues, dialyzed, and dried. The polyurethanes are extracted from VGs by dispersion/extraction in tetrahydrofuran (THF) and freed from the excess of THF-insoluble biopolymers. The stability of polyurethanes is assessed by IR spectroscopy and gel permeation chromatography. Pellethane has emerged to be stable at all experimental points. Tecoflex loses approximately 10% of its molecular weight (both Mn and Mw) after 3 months and restored its initial value within 6 months of its functioning as a graft. Mechanical testing demonstrates a 30% reduction in the tensile strength after 3 months in VG and a 10% increase after 6 months. The stability and mechanical properties of polyurethane-based VGs demonstrate their utility for the reconstitution of damaged arteries.

Alexander A Gostev , Shundrina I.K. , Pastukhov V.I. , Shutov A.V. , Vera S Chernonosova , Andrey A Karpenko , Laktionov P.P.
In Vivo Stability of Polyurethane-Based Electrospun Vascular Grafts in Terms of Chemistry and Mechanics
Polymers. 2020. V.12. N4. 845 :1-11. DOI: 10.3390/polym12040845 WOS РИНЦ OpenAlex

Submitted:	Mar 13, 2020
Accepted:	Mar 27, 2020
Published print:	Apr 7, 2020

Web of science:	WOS:000535587700115
Elibrary:	43305984
OpenAlex:	W3014554875

DB	Citing
OpenAlex	15
Elibrary	16
Web of science	16